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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB3877 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3877, RRID:AB_1579894
- Product name
- THY1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of THY1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Thy-1 expression in human fibroblast subsets defines myofibroblastic or lipofibroblastic phenotypes.
THY1 expression is associated with tumor suppression of human ovarian cancer.
The Thy-1/Thy-1 ligand interaction is involved in binding of melanoma cells to activated Thy-1- positive microvascular endothelial cells.
Koumas L, Smith TJ, Feldon S, Blumberg N, Phipps RP
The American journal of pathology 2003 Oct;163(4):1291-300
The American journal of pathology 2003 Oct;163(4):1291-300
THY1 expression is associated with tumor suppression of human ovarian cancer.
Abeysinghe HR, Cao Q, Xu J, Pollock S, Veyberman Y, Guckert NL, Keng P, Wang N
Cancer genetics and cytogenetics 2003 Jun;143(2):125-32
Cancer genetics and cytogenetics 2003 Jun;143(2):125-32
The Thy-1/Thy-1 ligand interaction is involved in binding of melanoma cells to activated Thy-1- positive microvascular endothelial cells.
Saalbach A, Hildebrandt G, Haustein UF, Anderegg U
Microvascular research 2002 Jul;64(1):86-93
Microvascular research 2002 Jul;64(1):86-93
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The THY1 polyclonal antibody (Cat # PAB3877) is used in Western blot to detect THY1 in HL-60 cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of THY1 polyclonal antibody (Cat # PAB3877) pre-incubated without (lane 1) and with (lane 2) blocking peptide in T-47D cell line lysate. THY1 (N-term) (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with THY1 polyclonal antibody (Cat # PAB3877) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
