PA5-47702

antibody from Invitrogen Antibodies

Targeting: TAFA5 FAM19A5, TAFA-5

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47702

Provider

Invitrogen Antibodies

Product name

TAFA5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TAFA1, rhTAFA2, rhTAFA3, and rhTAFA4 is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of bEnd.3 mouse endothelioma cell line, T98G human glioblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-human/mouse/Rat TAFA5/FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47702) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for TAFA5/FAM19A5 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TAFA5 in bEnd.3 mouse endothelioma cell line, T98G human glioblastoma cell line, Neuro‚2A mouse neuroblastoma cell line, and IMR‚32 human neuroblastoma cell line. Samples were incubated in TAFA5 polyclonal antibody (Product # PA5-47702) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for TAFA5/FAM19A5 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of IMR-32 human neuroblastoma cell line was stained with Goat Anti-human/mouse/Rat TAFA5/FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47702, filled histogram) or control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of TAFA5 in IMR‚32 human neuroblastoma cell line. Samples were incubated in TAFA5 polyclonal antibody (Product # PA5-47702) or control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.