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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF5148 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat TAFA5/FAM19A5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse TAFA5/FAM19A5 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TAFA1, rhTAFA2, rhTAFA3, and rhTAFA4 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_056196- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse TAFA5/FAM19A5 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line, T98G human glioblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat TAFA5/ FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TAFA5/FAM19A5 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TAFA5/FAM19A5 in IMR-32 Human Cell Line by Flow Cytometry. IMR-32 human neuro-blastoma cell line was stained with Goat Anti-Human/Mouse/Rat TAFA5/ FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
