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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- MA5-27591 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SYT10 Monoclonal Antibody (S269-73)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S269-73
- Vial size
- 100 µg
- Concentration
- 1 mg/ml
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SYT10 in rat brain lysates. The sample was incubated with SYT10 monoclonal antibody (Product # MA5-27591) using the following dilutions: 1:100, 1:250, 1:500 and 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SYT10 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with SYT10 monoclonal antibody (Product # MA5-27591) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Synaptotagmin-10 Antibody, D) Merged image. Magnification: 60x.
