Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA5-25841 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NMT2 Monoclonal Antibody (OTI2A5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2A5
- Vial size
- 100 µL
- Concentration
- 0.79 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular Lesions.
Govatati S, Pichavaram P, Janjanam J, Guo L, Virmani R, Rao GN
Arteriosclerosis, thrombosis, and vascular biology 2020 May;40(5):1256-1274
Arteriosclerosis, thrombosis, and vascular biology 2020 May;40(5):1256-1274
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NMT2 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with NMT2 (Product # MA5-25841) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NMT2 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with NMT2 (Product # MA5-25841) monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human lymphoma tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a NMT2 monoclonal antibody (Product # MA5-25841).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human tonsil tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a NMT2 monoclonal antibody (Product # MA5-25841).