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ImmunocytochemistryAntibody data
- Antibody Data
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- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00028951-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00028951-M04, RRID:AB_670655
- Product name
- Mouse Monoclonal TRIB2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. TRIB2 (1B1)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references High Glucose Stimulates Tumorigenesis in Hepatocellular Carcinoma Cells Through AGER-Dependent O-GlcNAcylation of c-Jun.
TRIB2 inhibits Wnt/β-Catenin/TCF4 signaling through its associated ubiquitin E3 ligases, β-TrCP, COP1 and Smurf1, in liver cancer cells.
Ubiquitin E3 ligase SCF(β-TRCP) regulates TRIB2 stability in liver cancer cells.
Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in liver cancer.
Qiao Y, Zhang X, Zhang Y, Wang Y, Xu Y, Liu X, Sun F, Wang J
Diabetes 2016 Mar;65(3):619-32
Diabetes 2016 Mar;65(3):619-32
TRIB2 inhibits Wnt/β-Catenin/TCF4 signaling through its associated ubiquitin E3 ligases, β-TrCP, COP1 and Smurf1, in liver cancer cells.
Xu S, Tong M, Huang J, Zhang Y, Qiao Y, Weng W, Liu W, Wang J, Sun F
FEBS letters 2014 Nov 28;588(23):4334-41
FEBS letters 2014 Nov 28;588(23):4334-41
Ubiquitin E3 ligase SCF(β-TRCP) regulates TRIB2 stability in liver cancer cells.
Qiao Y, Zhang Y, Wang J
Biochemical and biophysical research communications 2013 Nov 22;441(3):555-9
Biochemical and biophysical research communications 2013 Nov 22;441(3):555-9
Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in liver cancer.
Wang J, Zhang Y, Weng W, Qiao Y, Ma L, Xiao W, Yu Y, Pan Q, Sun F
The Journal of biological chemistry 2013 Nov 22;288(47):33667-33681
The Journal of biological chemistry 2013 Nov 22;288(47):33667-33681
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIB2 Antibody (1B1) [H00028951-M04] - TRIB2 expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIB2 Antibody (1B1) [H00028951-M04] - TRIB2 over-expressed 293 cell line, cotransfected with TRIB2 Validated Chimera RNAi or non-transfected control. Blot probed with H00028951-M04. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIB2 Antibody (1B1) [H00028951-M04] - Analysis of TRIB2 expression in transfected 293T cell line by TRIB2 monoclonal antibody (M04), clone 1B1. Lane 1: TRIB2 transfected lysatE (38.8 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TRIB2 Antibody (1B1) [H00028951-M04] - Detection limit for recombinant GST tagged TRIB2 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRIB2 Antibody (1B1) [H00028951-M04] - Analysis of monoclonal antibody to TRIB2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
