TA805076

antibody from OriGene

Targeting: GARS1 CMT2D, DSMAV, GARS, GlyRS, SMAD1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA805076

Provider

OriGene

Proper citation

OriGene Cat#TA805076, RRID:AB_2627576

Product name

GARS mouse monoclonal antibody, clone OTI4H8 (formerly 4H8)

Antibody type

Monoclonal

Description

GARS mouse monoclonal antibody, clone OTI4H8 (formerly 4H8)

Host

Mouse

Conjugate

Unconjugated

Epitope

GARS

Isotype

IgG

Antibody clone number

OTI4H8

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GARS (RC202852, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GARS.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GARS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805076)

Validation comment

IHC