Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 15831-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GARS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MRLGDAVEQ GVINNTVLGY FIGRIYLYLT KVGISPDKLR FRQHMENEMA HYACDCWDAE SKTSYGWIEI VGCADRSCYD LSCHARATKV PLVAEKPLKE PKTVNVVQFE PSKGAIGKAY KKDAKLVMEY LAICDECYIT EMEMLLNEKG EFTIETEGKT FQLTKDMINV KRFQKTLYVE EVVPNVIEPS FGLGRIMYTV FEHTFHVREG DEQRTFFSFP AVVAPFKCSV LPLSQNQEFM PFVKELSEAL TRHGVSHKVD DSSGSIGRRY ARTDEIGVAF GVTIDFDTVN KTPHTATLRD RDSMRQIRAE ISELPSIVQD LANGNITWAD VEARYPLFEG QETGKKETIE E (390-739 aa encoded by BC007755)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HT-1080 cells were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using GARS antibody 15831-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer using 15831-1-AP (GARS antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer using 15831-1-AP (GARS antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GARS (IP:15831-1-AP, 4ug; Detection:15831-1-AP 1:500) with mouse liver tissue lysate 4000ug.