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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-30065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HDJ2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (85%), Xenopus laevis (92%), Dog (100%), Pig (100%), Chicken (94%), Chimpanzee (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HDJ2 using 30 µg of A) Jurkat (B) Raji (C) THP-1 and D) HL-60 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a HDJ2 polyclonal antibody (Product # PA5-30065) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HDJ2 Polyclonal Antibody (Product # PA5-30065). Sample (30 µg of whole cell lysate). Lane A: Jurkat. Lane B: Raji. Lane C: THP-1. Lane D: HL-60. 10% SDS PAGE. HDJ2 Polyclonal Antibody (Product # PA5-30065) diluted at 1:1,000.
