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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP12029PU-N - Provider product page
- Provider
- OriGene
- Product name
- USP14 (N-term) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- USP14 (N-term) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- USP14
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- (LEFT) The anti-USP14 (N-term) Pab is used in Western blot to detect USP14 in USP14-transfected HeLa cell lysates. Transfection data is kindly provided by Dr. B. Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland). (RIGHT) Western blot analysis of anti-USP14 Pab (N-term) in A2058 cell line lysates (35ug/lane). USP14 (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
