Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB4199 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4199, RRID:AB_1581760
- Product name
- USP14 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of USP14.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Deubiquitination of CXCR4 by USP14 is critical for both CXCL12-induced CXCR4 degradation and chemotaxis but not ERK ativation.
Mines MA, Goodwin JS, Limbird LE, Cui FF, Fan GH
The Journal of biological chemistry 2009 Feb 27;284(9):5742-52
The Journal of biological chemistry 2009 Feb 27;284(9):5742-52
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The USP14 polyclonal antibody (Cat # PAB4199) is used in Western blot to detect USP14 in A-375 . Transfection data is kindly provided by Dr . B . Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP14 polyclonal antibody (Cat # PAB4199) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)