Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- sc-48351 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-48351, RRID:AB_626796
- Product name
- Anti-CA2
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Low levels of Na, K-ATPase and carbonic anhydrase II during choroid plexus development suggest limited involvement in early CSF secretion.
Characterization of CXCL16 and ADAM10 in the normal and transplanted kidney
Johansson P, Dziegielewska K, Saunders N
Neuroscience letters 2008 Sep 5;442(1):77-80
Neuroscience letters 2008 Sep 5;442(1):77-80
Characterization of CXCL16 and ADAM10 in the normal and transplanted kidney
Anja Schramme, Mohamed Sadek Abdel-Bakky, Paul Gutwein, Nicholas Obermüller, Patrick C Baer, Ingeborg A Hauser, Andreas Ludwig, Stefan Gauer, Liliana Schäfer, Ewelina Sobkowiak, Peter Altevogt, Michael Koziolek, Eva Kiss, Hermann-Josef Gröne, Ritva Tikkanen, Itamar Goren, Heinfried Radeke, Josef Pfeilschifter
Kidney International 2008 May;74(3):328-338
Kidney International 2008 May;74(3):328-338
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 29
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.