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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ABIN1449698 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Histone Cluster 3, H3 (HIST3H3) (acLys18) antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide derived from Human Histone H3 around the acetylation site of Lysine 18
- Description
- Immunoaffinity Chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antigen sequence
P-R-KA-Q-L- Epitope
- acLys18
- Isotype
- IgG
- Vial size
- 50 μg
- Concentration
- 1.0 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Nucleosome fractionation by mercury affinity chromatography. Contrasting distribution of transcriptionally active DNA sequences and acetylated histones in nucleosome fractions of wild-type yeast cells and cells expressing a histone H3 gene altered to encode a cysteine 110 residue.
Isolation and characterization of acetylated histones H3 and H4 and their assembly into nucleosomes.
Chen TA, Smith MM, Le SY, Sternglanz R, Allfrey VG
The Journal of biological chemistry 1991 Apr 5;266(10):6489-98
The Journal of biological chemistry 1991 Apr 5;266(10):6489-98
Isolation and characterization of acetylated histones H3 and H4 and their assembly into nucleosomes.
Marvin KW, Yau P, Bradbury EM
The Journal of biological chemistry 1990 Nov 15;265(32):19839-47
The Journal of biological chemistry 1990 Nov 15;265(32):19839-47
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HeLa cells, treated with TSA 400 nM 24h, using Histone H3 (Acetyl-Lys18) Antibody Cat.-No AP32267PU-N. The lane on the right is treated with the synthesized peptide.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys18) Antibody Cat.-No AP32267PU-N. The picture on the right is treated with the synthesized peptide.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Human, Breast epithelium: Formalin-Fixed Paraffin-Embedded (FFPE)
