PA5-48625

antibody from Invitrogen Antibodies

Targeting: H2AC21 HIST2H2AB

Western blot (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-48625

Provider

Invitrogen Antibodies

Product name

HIST2H2AB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Predicted to react with rat, mouse, bovine and chicken based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HIST2H2AB in ZR-75-1 cell line lysates (35 µg/lane). Lysates were probed with a HIST2H2AB Antibody (N-term) (Product # PA5-48625).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), MDA-MB-231 (Lane 3), COS-7 (Lane 4) and A549 (Lane 5). The blot was probed with Anti-HIST2H2AB Polyclonal Antibody (Product # PA5-48625, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 14 kDa band corresponding to HIST2H2AB was observed across the cell lines tested.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Enrichment of endogenous H3.3 protein at specific gene loci using Anti-H3.3 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone H3.3 Monoclonal Antibody (Product # MA5-24667, 3 µg) on sheared chromatin from 2 million U-2 OS cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs for the GAPDH PROMOTER, GAPDH EXON2 used as positive and MYOD, SAT2 satellite repeats, SAT Alpha used as negative target genes/binding sites. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.