Explore
Validate
Learn
Western blot
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A304-281A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAM83G Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human FAM83G by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-FAM83G antibody A304-281A (lot A304-281A-1) used for IP at 6 µg per reaction. FAM83G was also immunoprecipitated by rabbit anti-FAM83G antibody A304-282A. For blotting immunoprecipitated FAM83G, A304-282A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
