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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP18207PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP18207PU-N, RRID:AB_10653591
- Product name
- anti Spindlin 1 / SPIN1 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between aa 1-30 from the N-terminal region of human SPIN1.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of AP18207PU-N SPIN1 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). SPIN1 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Embedded Human testis tissue reacted with AP18207PU-N SPIN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP18207PU-N SPIN1 Antibody (N-term) Flow Cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
