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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA1-32133 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RFX5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Knockdown of LINC00504 Inhibits the Proliferation and Invasion of Breast Cancer via the Downregulation of miR-140-5p.
Hou T, Ye L, Wu S
OncoTargets and therapy 2021;14:3991-4003
OncoTargets and therapy 2021;14:3991-4003
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-RFX5 Polyclonal Antibody (Product # PA1-32133). Raji B cell nuclear extract lysates. Load: 35 µg per lane. Primary antibody: RFX5 Polyclonal Antibody at 1:2,500 for overnight at 4ºC. Secondary antibody: HRP Goat-a-Rabbit IgG at 1:5,000 for 45 min at RT.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 The key transcription factor RFX5 critically activated the transcription of LINC00504. ( A ) The expression of RFX5 in breast cancer patients and healthy subjects in The Cancer Genome Atlas (TCGA) database was assessed. ( B and D ) The expression level of RFX5 was detected in MDA-MB-231 and SKBR3 cells after transfection with RFX5 siRNAs or pcDNA-RFX5. ( C and E ) The effect of the altered expression level of RFX5 on the regulation of LINC00504. ( F ) ChIP-qPCR assays showing RFX5 binding to the LINC00504 promoter in BC cells. ( G and H ) ChIP-qPCR assays revealed the enrichment of RFX5 on the LINC00504 promoter in BC cells after transfection with RFX5 siRNA or pcDNA-RFX5. *P
