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LearnNBP1-88943
antibody from Novus Biologicals
Targeting: MIS18A
B28, C21orf45, C21orf46, FASP1, hMis18alpha
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- NBP1-88943 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88943, RRID:AB_11029449
- Product name
- Rabbit Polyclonal MIS18A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human MIS18A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MIS18A Antibody [NBP1-88943] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: NTERA-2 High
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: MIS18A Antibody [NBP1-88943] - Simple Western lane view shows a specific band for MIS18A in 0.2 mg/ml of Ntera-2 (left) and h. Testis (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: MIS18A Antibody [NBP1-88943] - Electropherogram image of the corresponding Simple Western lane view. MIS18A antibody was used at 1:25 dilution on Ntera-2 and h. Testis lysate(s) respectively.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MIS18A Antibody [NBP1-88943] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts. Sertoli cells are generally negative.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MIS18A Antibody [NBP1-88943] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MIS18A Antibody [NBP1-88943] - Staining in human testis and pancreas tissues using anti-MIS18A antibody. Corresponding MIS18A RNA-seq data are presented for the same tissues.
