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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- MAB12243 - Provider product page
- Provider
- Abnova Corporation
- Product name
- RAB27A monoclonal antibody, clone 7D7C9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant human RAB27A.
- Isotype
- IgG
- Antibody clone number
- 7D7C9
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human RAB27A (AA: 1-221) recombinant protein (Expected MW is 50.8 kDa) with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [RAB27A (AA: 1-221)-hIgGFc transfected HEK293 cells] with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:500-1:2000 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA analysis of RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:10000 dilution.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human prostate cancer tissues (A) and liver cancer tissues (B) with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:200-1:1000 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
