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LearnH00005873-M02
antibody from Novus Biologicals
Targeting: RAB27A
GS2, HsT18676, RAB27, RAM
Western blot ELISA
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00005873-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005873-M02, RRID:AB_2269303
- Product name
- Mouse Monoclonal Rab27a Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RAB27A - RAB27A, member RAS oncogene family
- Reactivity
- Human, Mouse, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references PML-RARa modulates the vascular signature of extracellular vesicles released by acute promyelocytic leukemia cells.
Exosome-mediated transfer of miR-222 is sufficient to increase tumor malignancy in melanoma.
Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.
Exosome-mediated microRNA signaling from breast cancer cells is altered by the anti-angiogenesis agent docosahexaenoic acid (DHA).
Supporting Role for GTPase Rab27a in Hepatitis C Virus RNA Replication through a Novel miR-122-Mediated Effect.
Activated Cdc42-bound IQGAP1 determines the cellular endocytic site.
Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.
Stepwise maturation of lytic granules during differentiation and activation of human CD8+ T lymphocytes.
Rab27b regulates exocytosis of secretory vesicles in acinar epithelial cells from the lacrimal gland.
Synaptotagmin-like protein 1 interacts with the GTPase-activating protein Rap1GAP2 and regulates dense granule secretion in platelets.
Immunohistochemical expression of rabphilin-3A-like (Noc2) in normal and tumor tissues of human endocrine pancreas.
The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines.
Fang Y, Garnier D, Lee TH, D'Asti E, Montermini L, Meehan B, Rak J
Angiogenesis 2016 Jan;19(1):25-38
Angiogenesis 2016 Jan;19(1):25-38
Exosome-mediated transfer of miR-222 is sufficient to increase tumor malignancy in melanoma.
Felicetti F, De Feo A, Coscia C, Puglisi R, Pedini F, Pasquini L, Bellenghi M, Errico MC, Pagani E, Carè A
Journal of translational medicine 2016 Feb 24;14:56
Journal of translational medicine 2016 Feb 24;14:56
Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.
Cetica V, Hackmann Y, Grieve S, Sieni E, Ciambotti B, Coniglio ML, Pende D, Gilmour K, Romagnoli P, Griffiths GM, Aricò M
The Journal of allergy and clinical immunology 2015 May;135(5):1310-8.e1
The Journal of allergy and clinical immunology 2015 May;135(5):1310-8.e1
Exosome-mediated microRNA signaling from breast cancer cells is altered by the anti-angiogenesis agent docosahexaenoic acid (DHA).
Hannafon BN, Carpenter KJ, Berry WL, Janknecht R, Dooley WC, Ding WQ
Molecular cancer 2015 Jul 16;14:133
Molecular cancer 2015 Jul 16;14:133
Supporting Role for GTPase Rab27a in Hepatitis C Virus RNA Replication through a Novel miR-122-Mediated Effect.
Chen TC, Hsieh CH, Sarnow P
PLoS pathogens 2015 Aug;11(8):e1005116
PLoS pathogens 2015 Aug;11(8):e1005116
Activated Cdc42-bound IQGAP1 determines the cellular endocytic site.
Kimura T, Yamaoka M, Taniguchi S, Okamoto M, Takei M, Ando T, Iwamatsu A, Watanabe T, Kaibuchi K, Ishizaki T, Niki I
Molecular and cellular biology 2013 Dec;33(24):4834-43
Molecular and cellular biology 2013 Dec;33(24):4834-43
Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.
Peinado H, Alečković M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, Hergueta-Redondo M, Williams C, García-Santos G, Ghajar C, Nitadori-Hoshino A, Hoffman C, Badal K, Garcia BA, Callahan MK, Yuan J, Martins VR, Skog J, Kaplan RN, Brady MS, Wolchok JD, Chapman PB, Kang Y, Bromberg J, Lyden D
Nature medicine 2012 Jun;18(6):883-91
Nature medicine 2012 Jun;18(6):883-91
Stepwise maturation of lytic granules during differentiation and activation of human CD8+ T lymphocytes.
Sanchez-Ruiz Y, Valitutti S, Dupre L
PloS one 2011;6(11):e27057
PloS one 2011;6(11):e27057
Rab27b regulates exocytosis of secretory vesicles in acinar epithelial cells from the lacrimal gland.
Chiang L, Ngo J, Schechter JE, Karvar S, Tolmachova T, Seabra MC, Hume AN, Hamm-Alvarez SF
American journal of physiology. Cell physiology 2011 Aug;301(2):C507-21
American journal of physiology. Cell physiology 2011 Aug;301(2):C507-21
Synaptotagmin-like protein 1 interacts with the GTPase-activating protein Rap1GAP2 and regulates dense granule secretion in platelets.
Neumüller O, Hoffmeister M, Babica J, Prelle C, Gegenbauer K, Smolenski AP
Blood 2009 Aug 13;114(7):1396-404
Blood 2009 Aug 13;114(7):1396-404
Immunohistochemical expression of rabphilin-3A-like (Noc2) in normal and tumor tissues of human endocrine pancreas.
Shanmugam C, Katkoori VR, Jhala NC, Grizzle WE, Manne U
Biotechnic & histochemistry : official publication of the Biological Stain Commission 2009 Apr;84(2):39-45
Biotechnic & histochemistry : official publication of the Biological Stain Commission 2009 Apr;84(2):39-45
The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines.
Kimura T, Kaneko Y, Yamada S, Ishihara H, Senda T, Iwamatsu A, Niki I
Journal of cell science 2008 Sep 15;121(Pt 18):3092-8
Journal of cell science 2008 Sep 15;121(Pt 18):3092-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rab27a Antibody (1G7) [H00005873-M02] - Analysis of RAB27A over-expressed 293 cell line, cotransfected with RAB27A Validated Chimera RNAi ( Cat # H00005873-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAB27A monoclonal antibody (M02), clone 1G7 (Cat # H00005873-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rab27a Antibody (1G7) [H00005873-M02] - Analysis of RAB27A expression in transfected 293T cell line by RAB27A monoclonal antibody (M02), clone 1G7.Lane 1: RAB27A transfected lysate(24.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rab27a Antibody (1G7) [H00005873-M02] - RAB27A monoclonal antibody (M02), clone 1G7 Analysis of RAB27A expression in HL-60.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Rab27a Antibody (1G7) [H00005873-M02] - Detection limit for recombinant GST tagged RAB27A is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rab27a Antibody (1G7) [H00005873-M02] - Analysis of monoclonal antibody to RAB27A on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 3 ug/ml.
