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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- M01608 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-RAB27A Antibody Picoband™ (monoclonal, 2F5)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for RAB27A detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2F5
- Vial size
- 100μg/vial
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of RAB27A using anti-RAB27A antibody (M01608). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates Lane 2: human HepG2 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human HT1080 whole cell lysates Lane 5: human SW620 whole cell lysates Lane 6: human PANC-1 whole cell lysates Lane 7: rat RH35 whole cell lysates Lane 8: mouse NIH/3T3 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RAB27A antigen affinity purified monoclonal antibody (Catalog # M01608) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RAB27A at approximately 27KD. The expected band size for RAB27A is at 27KD.
- Additional image
