LS-C747274

antibody from LSBio

Targeting: STAT1 ISGF-3, STAT91

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C747274

Provider

LSBio

Product name

STAT1 Antibody LS-C747274

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of HeLa cells, using STAT1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug STAT1 antibody. Western blot was performed from the immunoprecipitate using STAT1 antibody at a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug STAT1 antibody. Western blot was performed from the immunoprecipitate using STAT1 antibody at a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug STAT1 antibody. Western blot was performed from the immunoprecipitate using STAT1 antibody at a dilition of 1:1000.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human mammary cancer using STAT1 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gastric cancer using STAT1 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human mammary cancer using STAT1 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gastric cancer using STAT1 antibody at dilution of 1:100 (40x lens).