Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 627302 - Provider product page
- Provider
- BioLegend
- Product name
- Purified anti-STAT1
- Antibody type
- Monoclonal
- Antigen
- STAT1 peptide QLDSKFLEQVHQLYD
- Description
- STAT1, also known as signal transduction and activator of transcription-1, is a ubiquitously expressed cytoplasmic protein that becomes phosphorylated in response to cytokine signaling (interferon α, interferon γ, EGF, PDGF, and IL-6) by receptor-associated kinases and translocates to the nucleus to act as a transcriptional activator. Two isoforms of STAT1 exist as a result of alternative RNA processing with apparent molecular weights of 88 and 91 kD. STAT1 has been shown to interact with a number of proteins including STAT3, FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27 receptor, IL-2R β and γ, IFNαRβ, STAT2, c-Src, and many others. STAT1 knockout mice are highly sensitive to infection with microbial infections and viruses. The SAT-84 monoclonal antibody recognizes human STAT1 and has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4° C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Jurkat extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse monoclonal antibody against STAT1. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated