H00003217-M01

antibody from Abnova Corporation

Targeting: HOXB7 HOX2, HOX2C

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003217-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003217-M01, RRID:AB_565852

Product name

HOXB7 monoclonal antibody (M01), clone 4F9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant HOXB7.

Antigen sequence

MQGLYPGGGGMAGQSAAGVYAAGYGLEPSSFNMHC
APFEQNLSGVCPGDSAKAAGAKEQRDSDLAA


Isotype

IgG

Antibody clone number

4F9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Evidence for a functional role of epigenetically regulated midcluster HOXB genes in the development of Barrett esophagus.

di Pietro M, Lao-Sirieix P, Boyle S, Cassidy A, Castillo D, Saadi A, Eskeland R, Fitzgerald RC
Proceedings of the National Academy of Sciences of the United States of America 2012 Jun 5;109(23):9077-82

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Oncogenic HoxB7 requires TALE cofactors and is inactivated by a dominant-negative Pbx1 mutant in a cell-specific manner.

Fernandez LC, Errico MC, Bottero L, Penkov D, Resnati M, Blasi F, Caré A
Cancer letters 2008 Aug 8;266(2):144-55

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HOXB7 expression in transfected 293T cell line by HOXB7 monoclonal antibody (M01), clone 4F9.Lane 1: HOXB7 transfected lysate(23.97 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HOXB7 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol