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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX30366 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX30366, RRID:AB_385511
- Product name
- DNMT3B antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Keep as concentrated solution. For short-term storage, store at 4¢X C (up to 10 days). For long-term storage, aliquot and store at -20¢X C or below. Avoid multiple freeze-thaw cycles.
Submitted references DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells.
An essential role for DNA methyltransferase DNMT3B in cancer cell survival.
Robert MF, Morin S, Beaulieu N, Gauthier F, Chute IC, Barsalou A, MacLeod AR
Nature genetics 2003 Jan;33(1):61-5
Nature genetics 2003 Jan;33(1):61-5
An essential role for DNA methyltransferase DNMT3B in cancer cell survival.
Beaulieu N, Morin S, Chute IC, Robert MF, Nguyen H, MacLeod AR
The Journal of biological chemistry 2002 Aug 2;277(31):28176-81
The Journal of biological chemistry 2002 Aug 2;277(31):28176-81
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Detection of overexpressed DNMT-38 in HCT116 cells. Lane 1: 40 μg of nuclear extract from untransfected T24 degrees Cells. Lane 2-3: 40 μg of nuclear extracts transiently transfected to overexpress DNMT3B2 and DNMT3B3, respectively.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Dnmt3b Antibody [GTX30366] - Dnmt3b antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry: Dnmt3b Antibody [GTX30366] - Dnmt3b antibody was tested in mouse testis using DAB with hematoxylin counterstain.
