Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-301-H71 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-H71, RRID:AB_2611286
- Product name
- Anti-Dnmt3b (MOUSE) Monoclonal Antibody - 200-301-H71
- Antibody type
- Monoclonal
- Description
- Anti-Dnmt3b Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Dnmt3b from human and mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Dnmt3b from other sources has not been determined.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 52A1018
- Vial size
- 100 µl
- Concentration
- 0.5 mg/ml
- Storage
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Handling
- Dry Ice
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Mouse Anti-Dnmt3b antibody. Lane 1: Cell lysates of 293 transfected with mouse Dnmt3a. Lane B: Cell lysates of 293 transfected with mouse Dnmt3b. Load: 10 µg per lane. Primary antibody: Dnmt3a antibody at 2 µg/mL for overnight at 4°C against 10 µg of cell lysates. Secondary antibody: IRDye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 101.7 kDa for Dmnt3b in lane 2. Other band(s): none.
- Validation comment
- Western Blot