Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-38511 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNMT3B Monoclonal Antibody (4D1A2E5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody has been tested in indirect ELISA.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D1A2E5
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references DNMT1-Mediated DNA Methylation Targets CDKN2B to Promote the Repair of Retinal Ganglion Cells in Streptozotocin-Induced Mongolian Gerbils during Diabetic Retinopathy.
Wang X, Zhang J, Liao Y, Jin Y, Yu X, Li H, Yang Q, Li X, Chen R, Wu D, Zhu H
Computational and mathematical methods in medicine 2022;2022:9212116
Computational and mathematical methods in medicine 2022;2022:9212116
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DNMT3B using DNMT3B Monoclonal Antibody (Product # MA5-38511) in HEK293 (1) and DNMT3B (AA: 1-150)-human IgG-Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using DNMT3B Monoclonal Antibody (Product # MA5-38511) against human DNMT3B (AA: 1-150) recombinant protein. (Expected MW is 42.6 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DNMT3B using DNMT3B Monoclonal Antibody (Product # MA5-38511) in HEK293 (1) and DNMT3B (AA: 1-150)-human IgG-Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of DNMT3B in HeLa cells using DNMT3B Monoclonal Antibody (Product # MA5-38511) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of DNMT3B in K562 cells using DNMT3B Monoclonal Antibody (Product # MA5-38511) (green) and negative control (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 DNMT1-mediated DNA methylation was significantly increased in the early phase of diabetic retinopathy. (a) STZ injection upregulated the global DNA methylation level of the retina until 6 weeks after injection. (b) Quantification of the average 5-mC content in DNA methylation of the retina isolated from sodium citrate buffer injection (control) and STZ injection (STZ) groups. (c-d) Western blot showed the protein expression levels of DNMT1, DNMT3A, and DNMT3B. * P < 0.05, ** P < 0.01.
