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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- NB300-516 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-516, RRID:AB_10003018
- Product name
- Rabbit Polyclonal DNMT3B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This detects DNA methyltransferase 3b (Dnmt3b) from human and mouse tissues and cells as well as recombinant human Dnmt3b. This detects, to a lesser extent, full-length human recombinant Dnmt3a.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references MiR-30a and miR-379 modulate retinoic acid pathway by targeting DNA methyltransferase 3B in oral cancer.
GADD45A binds R-loops and recruits TET1 to CpG island promoters.
S-adenosylhomocysteine Hydrolase Participates in DNA Methylation Inheritance.
Metformin alters DNA methylation genome-wide via the H19/SAHH axis.
H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.
DNA demethylation-dependent AR recruitment and GATA factors drive Rhox5 homeobox gene transcription in the epididymis.
DNA methyltransferase 3B (DNMT3B) mutations in ICF syndrome lead to altered epigenetic modifications and aberrant expression of genes regulating development, neurogenesis and immune function.
LSH cooperates with DNA methyltransferases to repress transcription.
Shiah SG, Hsiao JR, Chang HJ, Hsu YM, Wu GH, Peng HY, Chou ST, Kuo CC, Chang JY
Journal of biomedical science 2020 Apr 2;27(1):46
Journal of biomedical science 2020 Apr 2;27(1):46
GADD45A binds R-loops and recruits TET1 to CpG island promoters.
Arab K, Karaulanov E, Musheev M, Trnka P, Schäfer A, Grummt I, Niehrs C
Nature genetics 2019 Feb;51(2):217-223
Nature genetics 2019 Feb;51(2):217-223
S-adenosylhomocysteine Hydrolase Participates in DNA Methylation Inheritance.
Ponnaluri VKC, Estève PO, Ruse CI, Pradhan S
Journal of molecular biology 2018 Jul 6;430(14):2051-2065
Journal of molecular biology 2018 Jul 6;430(14):2051-2065
Metformin alters DNA methylation genome-wide via the H19/SAHH axis.
Zhong T, Men Y, Lu L, Geng T, Zhou J, Mitsuhashi A, Shozu M, Maihle NJ, Carmichael GG, Taylor HS, Huang Y
Oncogene 2017 Apr 27;36(17):2345-2354
Oncogene 2017 Apr 27;36(17):2345-2354
H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.
Zhou J, Yang L, Zhong T, Mueller M, Men Y, Zhang N, Xie J, Giang K, Chung H, Sun X, Lu L, Carmichael GG, Taylor HS, Huang Y
Nature communications 2015 Dec 21;6:10221
Nature communications 2015 Dec 21;6:10221
DNA demethylation-dependent AR recruitment and GATA factors drive Rhox5 homeobox gene transcription in the epididymis.
Bhardwaj A, Song HW, Beildeck M, Kerkhofs S, Castoro R, Shanker S, De Gendt K, Suzuki K, Claessens F, Issa JP, Orgebin-Crist MC, Wilkinson MF
Molecular endocrinology (Baltimore, Md.) 2012 Apr;26(4):538-49
Molecular endocrinology (Baltimore, Md.) 2012 Apr;26(4):538-49
DNA methyltransferase 3B (DNMT3B) mutations in ICF syndrome lead to altered epigenetic modifications and aberrant expression of genes regulating development, neurogenesis and immune function.
Jin B, Tao Q, Peng J, Soo HM, Wu W, Ying J, Fields CR, Delmas AL, Liu X, Qiu J, Robertson KD
Human molecular genetics 2008 Mar 1;17(5):690-709
Human molecular genetics 2008 Mar 1;17(5):690-709
LSH cooperates with DNA methyltransferases to repress transcription.
Myant K, Stancheva I
Molecular and cellular biology 2008 Jan;28(1):215-26
Molecular and cellular biology 2008 Jan;28(1):215-26
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: DNMT3B Antibody [NB300-516] - Simple Western lane view shows a specific band for DNMT3B in 0.5 mg/mL of BG01V lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT3B Antibody [NB300-516] - Homogenized mouse brain cells. WB image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: DNMT3B Antibody [NB300-516] - Analysis of 25 ug of HeLa cell lysates and a molecular weight protein ladder.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse brain tissue (right) compared with a negative control in the absence of primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus of Human testis tissue (right) compared with a negative control in the absence of primary antibody (left).
