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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [43]
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- Western blot [5]
- Immunocytochemistry [1]
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- Product number
- GTX70224 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70224, RRID:AB_372445
- Product name
- NBS1 antibody [1D7]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
Submitted references Thymidylate kinase is critical for DNA repair via ATM-dependent Tip60 complex formation.
Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications.
NBS1 promotes the endonuclease activity of the MRE11-RAD50 complex by sensing CtIP phosphorylation.
Homeodomain Proteins Directly Regulate ATM Kinase Activity.
Restoration of Replication Fork Stability in BRCA1- and BRCA2-Deficient Cells by Inactivation of SNF2-Family Fork Remodelers.
Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response.
Interplay with the Mre11-Rad50-Nbs1 complex and phosphorylation by GSK3β implicate human B-Myb in DNA-damage signaling.
Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism.
Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma.
The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1γ sumoylation and poly-SUMO chain elongation.
Sam68/KHDRBS1 is critical for colon tumorigenesis by regulating genotoxic stress-induced NF-κB activation.
BMI1 attenuates etoposide-induced G2/M checkpoints via reducing ATM activation.
Proteomic analysis of ubiquitin-like posttranslational modifications induced by the adenovirus E4-ORF3 protein.
Direct activation of ATM by resveratrol under oxidizing conditions.
Effect of MRE11 loss on PARP-inhibitor sensitivity in endometrial cancer in vitro.
Mutations in the FHA-domain of ectopically expressed NBS1 lead to radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination.
Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1.
The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage.
PI3K Inhibition Augments the Therapeutic Efficacy of a 3a-aza-Cyclopenta[α]indene Derivative in Lung Cancer Cells.
Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex.
Nijmegen breakage syndrome: the clearance pathway for mutant nibrin protein is allele specific.
Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment.
Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1.
The CEACAM1 tumor suppressor is an ATM and p53-regulated gene required for the induction of cellular senescence by DNA damage.
An E2F1-mediated DNA damage response contributes to the replication of human cytomegalovirus.
Gender-specific response of brain corticosteroid receptors to stress and fluoxetine.
Ancient and recent adaptive evolution of primate non-homologous end joining genes.
Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response.
53BP1 promotes ATM activity through direct interactions with the MRN complex.
Ataxia telangiectasia mutated (ATM) inhibition transforms human mammary gland epithelial cells.
Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways.
The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats.
Clinical variability and expression of the NBN c.657del5 allele in Nijmegen Breakage Syndrome.
MRE11 complex links RECQ5 helicase to sites of DNA damage.
DNA ligase I and Nbs1 proteins associate in a complex and colocalize at replication factories.
DNA ligase I and Nbs1 proteins associate in a complex and colocalize at replication factories.
Rapid induction of chromatin-associated DNA mismatch repair proteins after MNNG treatment.
The NER protein Rad33 shows functional homology to human Centrin2 and is involved in modification of Rad4.
Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene.
Constitutive phosphorylation of MDC1 physically links the MRE11-RAD50-NBS1 complex to damaged chromatin.
Cancer incidence in Nijmegen breakage syndrome is modulated by the amount of a variant NBS protein.
Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks.
Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm.
Hu CM, Tsao N, Wang YT, Chen YJ, Chang ZF
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Feb;33(2):2017-2025
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Feb;33(2):2017-2025
Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications.
Sohn SY, Hearing P
mBio 2019 Feb 26;10(1)
mBio 2019 Feb 26;10(1)
NBS1 promotes the endonuclease activity of the MRE11-RAD50 complex by sensing CtIP phosphorylation.
Anand R, Jasrotia A, Bundschuh D, Howard SM, Ranjha L, Stucki M, Cejka P
The EMBO journal 2019 Apr 1;38(7)
The EMBO journal 2019 Apr 1;38(7)
Homeodomain Proteins Directly Regulate ATM Kinase Activity.
Johnson TE, Lee JH, Myler LR, Zhou Y, Mosley TJ, Yang SH, Bio-Bricks for Molecular Machines FRI Stream, Uprety N, Kim J, Paull TT
Cell reports 2018 Aug 7;24(6):1471-1483
Cell reports 2018 Aug 7;24(6):1471-1483
Restoration of Replication Fork Stability in BRCA1- and BRCA2-Deficient Cells by Inactivation of SNF2-Family Fork Remodelers.
Taglialatela A, Alvarez S, Leuzzi G, Sannino V, Ranjha L, Huang JW, Madubata C, Anand R, Levy B, Rabadan R, Cejka P, Costanzo V, Ciccia A
Molecular cell 2017 Oct 19;68(2):414-430.e8
Molecular cell 2017 Oct 19;68(2):414-430.e8
Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response.
Li Z, Li J, Kong Y, Yan S, Ahmad N, Liu X
Cancer research 2017 Jun 15;77(12):3169-3180
Cancer research 2017 Jun 15;77(12):3169-3180
Interplay with the Mre11-Rad50-Nbs1 complex and phosphorylation by GSK3β implicate human B-Myb in DNA-damage signaling.
Henrich SM, Usadel C, Werwein E, Burdova K, Janscak P, Ferrari S, Hess D, Klempnauer KH
Scientific reports 2017 Jan 27;7:41663
Scientific reports 2017 Jan 27;7:41663
Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism.
Reynolds JJ, Bicknell LS, Carroll P, Higgs MR, Shaheen R, Murray JE, Papadopoulos DK, Leitch A, Murina O, Tarnauskaitė Ž, Wessel SR, Zlatanou A, Vernet A, von Kriegsheim A, Mottram RM, Logan CV, Bye H, Li Y, Brean A, Maddirevula S, Challis RC, Skouloudaki K, Almoisheer A, Alsaif HS, Amar A, Prescott NJ, Bober MB, Duker A, Faqeih E, Seidahmed MZ, Al Tala S, Alswaid A, Ahmed S, Al-Aama JY, Altmüller J, Al Balwi M, Brady AF, Chessa L, Cox H, Fischetto R, Heller R, Henderson BD, Hobson E, Nürnberg P, Percin EF, Peron A, Spaccini L, Quigley AJ, Thakur S, Wise CA, Yoon G, Alnemer M, Tomancak P, Yigit G, Taylor AM, Reijns MA, Simpson MA, Cortez D, Alkuraya FS, Mathew CG, Jackson AP, Stewart GS
Nature genetics 2017 Apr;49(4):537-549
Nature genetics 2017 Apr;49(4):537-549
Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma.
Rasmussen RD, Gajjar MK, Jensen KE, Hamerlik P
Molecular oncology 2016 May;10(5):751-63
Molecular oncology 2016 May;10(5):751-63
The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1γ sumoylation and poly-SUMO chain elongation.
Sohn SY, Hearing P
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 14;113(24):6725-30
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 14;113(24):6725-30
Sam68/KHDRBS1 is critical for colon tumorigenesis by regulating genotoxic stress-induced NF-κB activation.
Fu K, Sun X, Wier EM, Hodgson A, Liu Y, Sears CL, Wan F
eLife 2016 Jul 25;5
eLife 2016 Jul 25;5
BMI1 attenuates etoposide-induced G2/M checkpoints via reducing ATM activation.
Wei F, Ojo D, Lin X, Wong N, He L, Yan J, Xu S, Major P, Tang D
Oncogene 2015 Jun 4;34(23):3063-75
Oncogene 2015 Jun 4;34(23):3063-75
Proteomic analysis of ubiquitin-like posttranslational modifications induced by the adenovirus E4-ORF3 protein.
Sohn SY, Bridges RG, Hearing P
Journal of virology 2015 Feb;89(3):1744-55
Journal of virology 2015 Feb;89(3):1744-55
Direct activation of ATM by resveratrol under oxidizing conditions.
Lee JH, Guo Z, Myler LR, Zheng S, Paull TT
PloS one 2014;9(6):e97969
PloS one 2014;9(6):e97969
Effect of MRE11 loss on PARP-inhibitor sensitivity in endometrial cancer in vitro.
Koppensteiner R, Samartzis EP, Noske A, von Teichman A, Dedes I, Gwerder M, Imesch P, Ikenberg K, Moch H, Fink D, Stucki M, Dedes KJ
PloS one 2014;9(6):e100041
PloS one 2014;9(6):e100041
Mutations in the FHA-domain of ectopically expressed NBS1 lead to radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination.
Ohara M, Funyu Y, Ebara S, Sakamoto Y, Seki R, Iijima K, Ohishi A, Kobayashi J, Komatsu K, Tachibana A, Tauchi H
Journal of radiation research 2014 Jul;55(4):690-8
Journal of radiation research 2014 Jul;55(4):690-8
Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1.
O'Sullivan RJ, Arnoult N, Lackner DH, Oganesian L, Haggblom C, Corpet A, Almouzni G, Karlseder J
Nature structural & molecular biology 2014 Feb;21(2):167-74
Nature structural & molecular biology 2014 Feb;21(2):167-74
The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage.
Larsen DH, Hari F, Clapperton JA, Gwerder M, Gutsche K, Altmeyer M, Jungmichel S, Toledo LI, Fink D, Rask MB, Grøfte M, Lukas C, Nielsen ML, Smerdon SJ, Lukas J, Stucki M
Nature cell biology 2014 Aug;16(8):792-803
Nature cell biology 2014 Aug;16(8):792-803
PI3K Inhibition Augments the Therapeutic Efficacy of a 3a-aza-Cyclopenta[α]indene Derivative in Lung Cancer Cells.
Sanjiv K, Chen CW, Kakadiya R, Tala S, Suman S, Wu MH, Chen YH, Su TL, Lee TC
Translational oncology 2014 Apr;7(2):256-266.e5
Translational oncology 2014 Apr;7(2):256-266.e5
Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex.
Lee JH, Mand MR, Deshpande RA, Kinoshita E, Yang SH, Wyman C, Paull TT
The Journal of biological chemistry 2013 May 3;288(18):12840-51
The Journal of biological chemistry 2013 May 3;288(18):12840-51
Nijmegen breakage syndrome: the clearance pathway for mutant nibrin protein is allele specific.
Salewsky B, Wessendorf P, Hirsch D, Krenzlin H, Digweed M
Gene 2013 May 1;519(2):217-21
Gene 2013 May 1;519(2):217-21
Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment.
Oplustilova L, Wolanin K, Mistrik M, Korinkova G, Simkova D, Bouchal J, Lenobel R, Bartkova J, Lau A, O'Connor MJ, Lukas J, Bartek J
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3837-50
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3837-50
Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1.
Moudry P, Lukas C, Macurek L, Neumann B, Heriche JK, Pepperkok R, Ellenberg J, Hodny Z, Lukas J, Bartek J
Cell death and differentiation 2012 May;19(5):798-807
Cell death and differentiation 2012 May;19(5):798-807
The CEACAM1 tumor suppressor is an ATM and p53-regulated gene required for the induction of cellular senescence by DNA damage.
Sappino AP, Buser R, Seguin Q, Fernet M, Lesne L, Gumy-Pause F, Reith W, Favaudon V, Mandriota SJ
Oncogenesis 2012 Apr 16;1(4):e7
Oncogenesis 2012 Apr 16;1(4):e7
An E2F1-mediated DNA damage response contributes to the replication of human cytomegalovirus.
E X, Pickering MT, Debatis M, Castillo J, Lagadinos A, Wang S, Lu S, Kowalik TF
PLoS pathogens 2011 May;7(5):e1001342
PLoS pathogens 2011 May;7(5):e1001342
Gender-specific response of brain corticosteroid receptors to stress and fluoxetine.
Elaković I, Djordjevic A, Adzic M, Djordjevic J, Radojčić M, Matić G
Brain research 2011 Apr 12;1384:61-8
Brain research 2011 Apr 12;1384:61-8
Ancient and recent adaptive evolution of primate non-homologous end joining genes.
Demogines A, East AM, Lee JH, Grossman SR, Sabeti PC, Paull TT, Sawyer SL
PLoS genetics 2010 Oct 21;6(10):e1001169
PLoS genetics 2010 Oct 21;6(10):e1001169
Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response.
Cha H, Lowe JM, Li H, Lee JS, Belova GI, Bulavin DV, Fornace AJ Jr
Cancer research 2010 May 15;70(10):4112-22
Cancer research 2010 May 15;70(10):4112-22
53BP1 promotes ATM activity through direct interactions with the MRN complex.
Lee JH, Goodarzi AA, Jeggo PA, Paull TT
The EMBO journal 2010 Feb 3;29(3):574-85
The EMBO journal 2010 Feb 3;29(3):574-85
Ataxia telangiectasia mutated (ATM) inhibition transforms human mammary gland epithelial cells.
Mandriota SJ, Buser R, Lesne L, Stouder C, Favaudon V, Maechler P, Béna F, Clément V, Rüegg C, Montesano R, Sappino AP
The Journal of biological chemistry 2010 Apr 23;285(17):13092-106
The Journal of biological chemistry 2010 Apr 23;285(17):13092-106
Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways.
Collaco RF, Bevington JM, Bhrigu V, Kalman-Maltese V, Trempe JP
Virology 2009 Sep 15;392(1):24-33
Virology 2009 Sep 15;392(1):24-33
The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats.
Adzic M, Djordjevic A, Demonacos C, Krstic-Demonacos M, Radojcic MB
The international journal of biochemistry & cell biology 2009 Nov;41(11):2181-8
The international journal of biochemistry & cell biology 2009 Nov;41(11):2181-8
Clinical variability and expression of the NBN c.657del5 allele in Nijmegen Breakage Syndrome.
Lins S, Kim R, Krüger L, Chrzanowska KH, Seemanova E, Digweed M
Gene 2009 Nov 1;447(1):12-7
Gene 2009 Nov 1;447(1):12-7
MRE11 complex links RECQ5 helicase to sites of DNA damage.
Zheng L, Kanagaraj R, Mihaljevic B, Schwendener S, Sartori AA, Gerrits B, Shevelev I, Janscak P
Nucleic acids research 2009 May;37(8):2645-57
Nucleic acids research 2009 May;37(8):2645-57
DNA ligase I and Nbs1 proteins associate in a complex and colocalize at replication factories.
Vago R, Leva V, Biamonti G, Montecucco A
Cell cycle (Georgetown, Tex.) 2009 Aug 15;8(16):2600-7
Cell cycle (Georgetown, Tex.) 2009 Aug 15;8(16):2600-7
DNA ligase I and Nbs1 proteins associate in a complex and colocalize at replication factories.
Vago R, Leva V, Biamonti G, Montecucco A
Cell cycle (Georgetown, Tex.) 2009 Aug 15;8(16):2600-7
Cell cycle (Georgetown, Tex.) 2009 Aug 15;8(16):2600-7
Rapid induction of chromatin-associated DNA mismatch repair proteins after MNNG treatment.
Schroering AG, Williams KJ
DNA repair 2008 Jun 1;7(6):951-69
DNA repair 2008 Jun 1;7(6):951-69
The NER protein Rad33 shows functional homology to human Centrin2 and is involved in modification of Rad4.
den Dulk B, van Eijk P, de Ruijter M, Brandsma JA, Brouwer J
DNA repair 2008 Jun 1;7(6):858-68
DNA repair 2008 Jun 1;7(6):858-68
Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene.
Bartkova J, Tommiska J, Oplustilova L, Aaltonen K, Tamminen A, Heikkinen T, Mistrik M, Aittomäki K, Blomqvist C, Heikkilä P, Lukas J, Nevanlinna H, Bartek J
Molecular oncology 2008 Dec;2(4):296-316
Molecular oncology 2008 Dec;2(4):296-316
Constitutive phosphorylation of MDC1 physically links the MRE11-RAD50-NBS1 complex to damaged chromatin.
Spycher C, Miller ES, Townsend K, Pavic L, Morrice NA, Janscak P, Stewart GS, Stucki M
The Journal of cell biology 2008 Apr 21;181(2):227-40
The Journal of cell biology 2008 Apr 21;181(2):227-40
Cancer incidence in Nijmegen breakage syndrome is modulated by the amount of a variant NBS protein.
Krüger L, Demuth I, Neitzel H, Varon R, Sperling K, Chrzanowska KH, Seemanova E, Digweed M
Carcinogenesis 2007 Jan;28(1):107-11
Carcinogenesis 2007 Jan;28(1):107-11
Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks.
Takemura H, Rao VA, Sordet O, Furuta T, Miao ZH, Meng L, Zhang H, Pommier Y
The Journal of biological chemistry 2006 Oct 13;281(41):30814-23
The Journal of biological chemistry 2006 Oct 13;281(41):30814-23
Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm.
Cerosaletti K, Concannon P
The Journal of biological chemistry 2004 Sep 10;279(37):38813-9
The Journal of biological chemistry 2004 Sep 10;279(37):38813-9
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1D7] (GTX70224) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 7.5% SDS PAGE GTX70224 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Detection of human p95/NBS1 protein expression by anti-p95/NBS1 1D7 monoclonal antibody (GTX70224) in western blot experiment.Sample: (A) HeLa and (B) HepG2 whole cell extract.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1D7] (GTX70224) diluted at1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1D7] (GTX70224) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa, using NBS1(GTX70224) antibody at 1:100 dilution.
