UM800029

antibody from OriGene

Targeting: NBN AT-V1, AT-V2, ATV, NBS, NBS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

UM800029

Provider

OriGene

Proper citation

OriGene Cat#UM800029, RRID:AB_2629141

Product name

NBN mouse monoclonal antibody,clone UMAB99

Antibody type

Monoclonal

Description

NBN mouse monoclonal antibody,clone UMAB99

Host

Mouse

Conjugate

Unconjugated

Epitope

NBN

Isotype

IgG

Antibody clone number

UMAB99

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NBN monoclonal antibody (Clone UMAB99).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of A549 cells using NBN mouse monoclonal antibody (UM800029, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-NBN mouse monoclonal antibody (UM800029, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-$NBNmouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Validation comment

IHC

Other assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NBN mouse monoclonal antibody (UUM800029). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Validation comment

10K-CHIP