M00732

antibody from Boster Biological Technology

Targeting: NBN AT-V1, AT-V2, ATV, NBS, NBS1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00732

Provider

Boster Biological Technology

Product name

Anti-p95/NBS1 NBN Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Nbs1/NBN detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat Nbs1/NBN information: Molecular Weight: 84959 MW; Subcellular Localization: Nucleus . Nucleus, PML body . Chromosome, telomere . Localizes to discrete nuclear foci after treatment with genotoxic agents; Tissue Specificity: Ubiquitous. Expressed at high levels in testis.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

COF-14

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rat heart, using p95/NBS1 Antibody(M00732)NBN was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NBN Antibody (M00732)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image