Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-38579 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NBS1 Monoclonal Antibody (7E4A2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody has been tested in indirect ELISA.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7E4A2
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NBS1 using NBS1 Monoclonal Antibody (Product # MA5-38579) in A549 (1), Jurkat (2) and PC-12 (3) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using NBS1 Monoclonal Antibody (Product # MA5-38579) against human NBN (AA: 467-615) recombinant protein. (Expected MW is 44.3 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NBS1 using NBS1 Monoclonal Antibody (Product # MA5-38579) in HEK293 (1) and NBN (AA: 467-615)-human IgG-Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of NBS1 in HeLa cells using NBS1 Monoclonal Antibody (Product # MA5-38579) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Product # 35503)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NBS1 in paraffin-embedded cervical cancer tissues using NBS1 Monoclonal Antibody (Product # MA5-38579) and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NBS1 in paraffin-embedded rectum cancer tissues using NBS1 Monoclonal Antibody (Product # MA5-38579) and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of NBS1 in HeLa cells using NBS1 Monoclonal Antibody (Product # MA5-38579) (green) and negative control (red).