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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- NB100-221 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-221, RRID:AB_10001212
- Product name
- Mouse Monoclonal Nbs1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Nijmegen breakage syndrome (NBS, nibrin) is an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. Nbs1 Antibody (1D7) recognizes the 95kDa NBS protein which contains a fork FHA head-associated domain that is adjacent to BRCT, a breast cancer C terminal domain, that has been implicated in protein-protein interactions. NBS is member of the Mre11/Rad50, p400 double-strand break repair complex involved in double-strand break repair and cell-cycle checkpoint functions.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references PPARγ Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis.
Identification of Sestrin3 Involved in the In vitro Resistance of Colorectal Cancer Cells to Irinotecan.
Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1.
An E2F1-mediated DNA damage response contributes to the replication of human cytomegalovirus.
Activated sterol regulatory element-binding protein-2 suppresses hepatocyte nuclear factor-4-mediated Cyp3a11 expression in mouse liver.
Ancient and recent adaptive evolution of primate non-homologous end joining genes.
Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response.
53BP1 promotes ATM activity through direct interactions with the MRN complex.
Ataxia telangiectasia mutated (ATM) inhibition transforms human mammary gland epithelial cells.
Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways.
Clinical variability and expression of the NBN c.657del5 allele in Nijmegen Breakage Syndrome.
MRE11 complex links RECQ5 helicase to sites of DNA damage.
The NER protein Rad33 shows functional homology to human Centrin2 and is involved in modification of Rad4.
Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene.
Constitutive phosphorylation of MDC1 physically links the MRE11-RAD50-NBS1 complex to damaged chromatin.
Cancer incidence in Nijmegen breakage syndrome is modulated by the amount of a variant NBS protein.
Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks.
Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm.
Li CG, Mahon C, Sweeney NM, Verschueren E, Kantamani V, Li D, Hennigs JK, Marciano DP, Diebold I, Abu-Halawa O, Elliott M, Sa S, Guo F, Wang L, Cao A, Guignabert C, Sollier J, Nickel NP, Kaschwich M, Cimprich KA, Rabinovitch M
Cell reports 2019 Jan 29;26(5):1333-1343.e7
Cell reports 2019 Jan 29;26(5):1333-1343.e7
Identification of Sestrin3 Involved in the In vitro Resistance of Colorectal Cancer Cells to Irinotecan.
Choi SH, Hong HK, Cho YB, Lee WY, Yoo HY
PloS one 2015;10(5):e0126830
PloS one 2015;10(5):e0126830
Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1.
Moudry P, Lukas C, Macurek L, Neumann B, Heriche JK, Pepperkok R, Ellenberg J, Hodny Z, Lukas J, Bartek J
Cell death and differentiation 2012 May;19(5):798-807
Cell death and differentiation 2012 May;19(5):798-807
An E2F1-mediated DNA damage response contributes to the replication of human cytomegalovirus.
E X, Pickering MT, Debatis M, Castillo J, Lagadinos A, Wang S, Lu S, Kowalik TF
PLoS pathogens 2011 May;7(5):e1001342
PLoS pathogens 2011 May;7(5):e1001342
Activated sterol regulatory element-binding protein-2 suppresses hepatocyte nuclear factor-4-mediated Cyp3a11 expression in mouse liver.
Inoue S, Yoshinari K, Sugawara M, Yamazoe Y
Molecular pharmacology 2011 Jan;79(1):148-56
Molecular pharmacology 2011 Jan;79(1):148-56
Ancient and recent adaptive evolution of primate non-homologous end joining genes.
Demogines A, East AM, Lee JH, Grossman SR, Sabeti PC, Paull TT, Sawyer SL
PLoS genetics 2010 Oct 21;6(10):e1001169
PLoS genetics 2010 Oct 21;6(10):e1001169
Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response.
Cha H, Lowe JM, Li H, Lee JS, Belova GI, Bulavin DV, Fornace AJ Jr
Cancer research 2010 May 15;70(10):4112-22
Cancer research 2010 May 15;70(10):4112-22
53BP1 promotes ATM activity through direct interactions with the MRN complex.
Lee JH, Goodarzi AA, Jeggo PA, Paull TT
The EMBO journal 2010 Feb 3;29(3):574-85
The EMBO journal 2010 Feb 3;29(3):574-85
Ataxia telangiectasia mutated (ATM) inhibition transforms human mammary gland epithelial cells.
Mandriota SJ, Buser R, Lesne L, Stouder C, Favaudon V, Maechler P, Béna F, Clément V, Rüegg C, Montesano R, Sappino AP
The Journal of biological chemistry 2010 Apr 23;285(17):13092-106
The Journal of biological chemistry 2010 Apr 23;285(17):13092-106
Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways.
Collaco RF, Bevington JM, Bhrigu V, Kalman-Maltese V, Trempe JP
Virology 2009 Sep 15;392(1):24-33
Virology 2009 Sep 15;392(1):24-33
Clinical variability and expression of the NBN c.657del5 allele in Nijmegen Breakage Syndrome.
Lins S, Kim R, Krüger L, Chrzanowska KH, Seemanova E, Digweed M
Gene 2009 Nov 1;447(1):12-7
Gene 2009 Nov 1;447(1):12-7
MRE11 complex links RECQ5 helicase to sites of DNA damage.
Zheng L, Kanagaraj R, Mihaljevic B, Schwendener S, Sartori AA, Gerrits B, Shevelev I, Janscak P
Nucleic acids research 2009 May;37(8):2645-57
Nucleic acids research 2009 May;37(8):2645-57
The NER protein Rad33 shows functional homology to human Centrin2 and is involved in modification of Rad4.
den Dulk B, van Eijk P, de Ruijter M, Brandsma JA, Brouwer J
DNA repair 2008 Jun 1;7(6):858-68
DNA repair 2008 Jun 1;7(6):858-68
Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene.
Bartkova J, Tommiska J, Oplustilova L, Aaltonen K, Tamminen A, Heikkinen T, Mistrik M, Aittomäki K, Blomqvist C, Heikkilä P, Lukas J, Nevanlinna H, Bartek J
Molecular oncology 2008 Dec;2(4):296-316
Molecular oncology 2008 Dec;2(4):296-316
Constitutive phosphorylation of MDC1 physically links the MRE11-RAD50-NBS1 complex to damaged chromatin.
Spycher C, Miller ES, Townsend K, Pavic L, Morrice NA, Janscak P, Stewart GS, Stucki M
The Journal of cell biology 2008 Apr 21;181(2):227-40
The Journal of cell biology 2008 Apr 21;181(2):227-40
Cancer incidence in Nijmegen breakage syndrome is modulated by the amount of a variant NBS protein.
Krüger L, Demuth I, Neitzel H, Varon R, Sperling K, Chrzanowska KH, Seemanova E, Digweed M
Carcinogenesis 2007 Jan;28(1):107-11
Carcinogenesis 2007 Jan;28(1):107-11
Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks.
Takemura H, Rao VA, Sordet O, Furuta T, Miao ZH, Meng L, Zhang H, Pommier Y
The Journal of biological chemistry 2006 Oct 13;281(41):30814-23
The Journal of biological chemistry 2006 Oct 13;281(41):30814-23
Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm.
Cerosaletti K, Concannon P
The Journal of biological chemistry 2004 Sep 10;279(37):38813-9
The Journal of biological chemistry 2004 Sep 10;279(37):38813-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nbs1 Antibody (1D7) [NB100-221] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nbs1 Antibody (1D7) [NB100-221] diluted at1:500. The HRP-conjugated anti-mouse IgG antibody [NBP2-19382] was used to detect the primary antibody. Observed Molecular Weight at ~95 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nbs1 Antibody (1D7) [NB100-221] - Sample (30 ug of whole cell lysate). A: HepG2. 7.5% SDS PAGE. Nbs1 Antibody (1D7) [NB100-221] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. Observed Molecular Weight at ~97 kDa.
