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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-30024 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Complement Component C1qC Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Complement Component C1qC Antibody [NBP2-30024] - Western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the C1QC antibody detected the C1QC protein (arrow).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Complement Component C1qC Antibody [NBP2-30024] - Confocal immunofluorescent analysis of C1QC Antibody (Center)(NBP2-30024) with WiDr cell followed by Alexa Fluor(R) 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Complement Component C1qC Antibody [NBP2-30024] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of C1QC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Complement Component C1qC Antibody [NBP2-30024] - Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
