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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- NB200-540 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-540, RRID:AB_10003444
- Product name
- Rat Monoclonal Complement C3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Mouse Complement C3 and its activation products, C3b, iC3b, C3d and C3dg
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth.
Autotransporter-Mediated Display of Complement Receptor Ligands by Gram-Negative Bacteria Increases Antibody Responses and Limits Disease Severity.
Rat IgG mediated circulatory cell depletion in mice requires mononuclear phagocyte system and is facilitated by complement.
GSPE alleviates renal fibrosis by inhibiting the activation of C3/ HMGB1/ TGF-β1 pathway.
Potassium and glutamate transport is impaired in scar-forming tumor-associated astrocytes.
Complement C3 Is Activated in Human AD Brain and Is Required for Neurodegeneration in Mouse Models of Amyloidosis and Tauopathy.
Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy.
Circulating AIM prevents hepatocellular carcinoma through complement activation.
Davidson S, Efremova M, Riedel A, Mahata B, Pramanik J, Huuhtanen J, Kar G, Vento-Tormo R, Hagai T, Chen X, Haniffa MA, Shields JD, Teichmann SA
Cell reports 2020 May 19;31(7):107628
Cell reports 2020 May 19;31(7):107628
Autotransporter-Mediated Display of Complement Receptor Ligands by Gram-Negative Bacteria Increases Antibody Responses and Limits Disease Severity.
Holland-Tummillo KM, Shoudy LE, Steiner D, Kumar S, Rosa SJ, Namjoshi P, Singh A, Sellati TJ, Gosselin EJ, Hazlett KR
Pathogens (Basel, Switzerland) 2020 May 14;9(5)
Pathogens (Basel, Switzerland) 2020 May 14;9(5)
Rat IgG mediated circulatory cell depletion in mice requires mononuclear phagocyte system and is facilitated by complement.
Sun D, Sun P, He S, Shi M
Journal of leukocyte biology 2020 Mar;107(3):529-539
Journal of leukocyte biology 2020 Mar;107(3):529-539
GSPE alleviates renal fibrosis by inhibiting the activation of C3/ HMGB1/ TGF-β1 pathway.
Wang K, Wei H, Zhan J, Liang X, Zhang C, Liu Y, Xu G
Chemico-biological interactions 2020 Jan 25;316:108926
Chemico-biological interactions 2020 Jan 25;316:108926
Potassium and glutamate transport is impaired in scar-forming tumor-associated astrocytes.
Campbell SC, Muñoz-Ballester C, Chaunsali L, Mills WA 3rd, Yang JH, Sontheimer H, Robel S
Neurochemistry international 2020 Feb;133:104628
Neurochemistry international 2020 Feb;133:104628
Complement C3 Is Activated in Human AD Brain and Is Required for Neurodegeneration in Mouse Models of Amyloidosis and Tauopathy.
Wu T, Dejanovic B, Gandham VD, Gogineni A, Edmonds R, Schauer S, Srinivasan K, Huntley MA, Wang Y, Wang TM, Hedehus M, Barck KH, Stark M, Ngu H, Foreman O, Meilandt WJ, Elstrott J, Chang MC, Hansen DV, Carano RAD, Sheng M, Hanson JE
Cell reports 2019 Aug 20;28(8):2111-2123.e6
Cell reports 2019 Aug 20;28(8):2111-2123.e6
Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy.
Medler TR, Murugan D, Horton W, Kumar S, Cotechini T, Forsyth AM, Leyshock P, Leitenberger JJ, Kulesz-Martin M, Margolin AA, Werb Z, Coussens LM
Cancer cell 2018 Oct 8;34(4):561-578.e6
Cancer cell 2018 Oct 8;34(4):561-578.e6
Circulating AIM prevents hepatocellular carcinoma through complement activation.
Maehara N, Arai S, Mori M, Iwamura Y, Kurokawa J, Kai T, Kusunoki S, Taniguchi K, Ikeda K, Ohara O, Yamamura KI, Miyazaki T
Cell reports 2014 Oct 9;9(1):61-74
Cell reports 2014 Oct 9;9(1):61-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This representative photomicrograph shows a membrane-cytoplasmic immunopositivity in non-germinal center cells, and few cells developed an intense staining for this target protein.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow cytometry. The C3b+ population of CD45+ cells is what the gate in each sample is exhibiting. Image from verified customer review.
