Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 846402 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#846402, RRID:AB_2572175
- Product name
- Purified anti-complement C3/C3b/iC3b
- Antibody type
- Monoclonal
- Antigen
- Human iC3b coupled to sepharose 4B
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 7C12/C3b
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot of clone 7C12/C3b. Lane 1: Molecular weight marker; Lane 2: 2 µg C3 recombinant protein; Lane 3: 2 µg C3b recombinant protein; Lane 4 and 5: Human Plasma; Lane 6: BSA negative control. Blot was incubated with clone 7C12/C3b at 10 µg/ml for 24 hours at 4°C. Following primary antibody, the blot was incubated with fluorescent labeled goat anti-mouse secondary antibody.
- Conjugate
- Unconjugated