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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-32080 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32080, RRID:AB_10003900
- Product name
- Rabbit Polyclonal Complement C3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Specific contribution of mannose-binding lectin murine isoforms to brain ischemia/reperfusion injury.
Neglia L, Oggioni M, Mercurio D, De Simoni MG, Fumagalli S
Cellular & molecular immunology 2020 Mar;17(3):218-226
Cellular & molecular immunology 2020 Mar;17(3):218-226
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Complement C3 Antibody [NBP1-32080] - Simple Western lane view shows a specific band for Complement C3 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at a dilution of 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Complement C3 Antibody [NBP1-32080] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Complement C3 Antibody [NBP1-32080] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Complement C3 Antibody [NBP1-32080] - Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 ug of C3 antibody [C3], C-term.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Paraffin-embedded mouse brain diluted at 1:500.
