AF1930

antibody from R&D Systems

Targeting: PTPN2 PTPT, TC-PTP, TCELLPTP, TCPTP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1930

Provider

R&D Systems

Product name

Human/Mouse/Rat TC-PTP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse and rat TC-PTP in Western blots.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P17706


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Integrin {alpha}1{beta}1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase.

Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A
The Journal of biological chemistry 2010 Dec 17;285(51):40114-24

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human TC-PTP by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for TC-PTP at approximately 51 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat TC-PTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1930) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat TC-PTP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TC-PTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1930) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TC-PTP at approximately 45 to 48 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.