MAB1930

antibody from R&D Systems

Targeting: PTPN2 PTPT, TC-PTP, TCELLPTP, TCPTP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1930

Provider

R&D Systems

Product name

Human/Mouse/Rat TC-PTP Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat TC-PTP in Western blots. In Western blots, no cross-reactivity with PTP1B is observed.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P17706


Isotype

IgG

Antibody clone number

252294

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development.

Lee H, Kim M, Baek M, Morales LD, Jang IS, Slaga TJ, DiGiovanni J, Kim DJ
Scientific reports 2017 Mar 21;7:45077

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Phosphorylation at tyrosine 262 promotes GADD34 protein turnover.

Zhou W, Jeyaraman K, Yusoff P, Shenolikar S
The Journal of biological chemistry 2013 Nov 15;288(46):33146-55

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STAT1 is a master regulator of pancreatic {beta}-cell apoptosis and islet inflammation.

Moore F, Naamane N, Colli ML, Bouckenooghe T, Ortis F, Gurzov EN, Igoillo-Esteve M, Mathieu C, Bontempi G, Thykjaer T, Ørntoft TF, Eizirik DL
The Journal of biological chemistry 2011 Jan 14;286(2):929-41

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Integrin {alpha}1{beta}1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase.

Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A
The Journal of biological chemistry 2010 Dec 17;285(51):40114-24

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat TC-PTP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat TC-PTP Monoclonal Antibody (Catalog # MAB1930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and Recombinant Human TC-PTP aa 2-314 (Catalog # 1930-PT) (1 ng/lane) were included. A specific band for TC-PTP was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.