PA5-42001

antibody from Invitrogen Antibodies

Targeting: C4BPA C4BP

Provider product page for PA5-42001

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [2]
Comments [0]
Validations
Western blot [3]
Other assay [1]

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Product number: PA5-42001 - Provider product page
Provider: Invitrogen Antibodies
Product name: C4BPA Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Peptide sequence: QVLTGKRLMQ CLPNPEDVKM ALEVYKLSLE IEQLELQRDS ARQSTLDKEL
Concentration: 0.5 mg/mL

C4BPA protein structure - PA5-42001 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HepG2 whole cell lysate using an anti-C4BP polyclonal antibody (Product # PA5-42001). Lane 1) Primary Antibody; Lane 2) Primary Antibody + Blocking Peptide.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of human MCF-7 cell lysate using an anti-C4BP polyclonal antibody (Product # PA5-42001).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-C4BPA Polyclonal Antibody (Product # PA5-42001) and bands corresponding to 93kDa and 52kDa of C4BPA were observed in the reported high expressing models- Hep G2, Mouse Epididymis, Mouse Liver, Rat Epididymis and Rat Liver in comparison to DU 145, U-937, BeWO and Mouse Lung . Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), DU 145 (Lane 2), U-937 (Lane 3), BeWO (Lane 4), tissue extracts (30ug lysate) of Mouse Lungs (Lane 4), Mouse Epididymis (Lane 5), Mouse Liver (Lane 6), Rat Epididymis (Lane 7) and Rat Liver (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001).The blot was probed with the antibody (0.75 µg/ml) and detected by ECL with Goat anti-Rabbit IgG (H+L), Superclonal™ secondary, HRP (Product # A27036, 1:4000 dilution). ECL was performed using Novex® ECL Kit (Product # WP20005). C4 protein, composed of alpha, beta and gamma chain, forms a heterotrimer of ~93 kDa alpha chains via disulphide linkage as detected by this antibody and a ~52 kDa protein corresponding to mouse and rat specific isoforms of C4BPA. It is also reported to be secreted by the epithelial cells surrounding the epididymis for sperm maturation in a region-specific, androgen dependent and species specific manner in addition to being synthesized in the liver.