Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C375972 - Provider product page
- Provider
- LSBio
- Product name
- IRAK4 / IRAK-4 Antibody (aa1-220) LS-C375972
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot. All lanes: Interleukin-1 receptor-associated kinase 4 antibody at 4 ug/ml+Jurkat whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 51 kDa. Observed band size: 51 kDa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All lanes: Interleukin-1 receptor-associated kinase 4 antibody at 4µg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 52, 38 kDa Observed band size: 52 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating IRAK4 in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating IRAK4 in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating IRAK4 in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (10µg)