A00042-1

antibody from Boster Biological Technology

Targeting: CASP8 Casp-8, FLICE, MACH, MCH5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

A00042-1

Provider

Boster Biological Technology

Product name

Anti-Caspase 8/CASP8 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Caspase 8 detection. Tested with WB, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Regulatory role of oxidative stress in retrorsine - Induced apoptosis and autophagy in primary rat hepatocytes.

Zhu Y, Zhang S, Shao Y, Tang L, Zhang C, Tang S, Lu H
Ecotoxicology and environmental safety 2024 Jul 1;279:116515

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Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway.

Fu X, Mao Q, Zhang B, Lv J, Ping K, Zhang P, Lin F, Zhao J, Feng Y, Yang J, Wang H, Zhang L, Mou Y, Wang S
Journal of natural products 2022 Apr 22;85(4):1147-1156

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Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs.

Guan YQ, Li Z, Chen J, Tao H, Wang W, Zheng Z, Li L, Liu JM
Biomaterials 2011 May;32(14):3637-46

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Caspase 8 using anti-Caspase 8 antibody (A00042-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 8 antigen affinity purified polyclonal antibody (Catalog # A00042-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 8 at approximately 55KD. The expected band size for Caspase 8 is at 55KD.

Additional image