PAB3419

antibody from Abnova Corporation

Targeting: BTK AGMX1, ATK, IMD1, PSCTK1, XLA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3419

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3419, RRID:AB_1571800

Product name

BTK polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of BTK.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering.

Márquez JA, Smith CI, Petoukhov MV, Lo Surdo P, Mattsson PT, Knekt M, Westlund A, Scheffzek K, Saraste M, Svergun DI
The EMBO journal 2003 Sep 15;22(18):4616-24

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Bruton's tyrosine kinase is required for lipopolysaccharide-induced tumor necrosis factor alpha production.

Horwood NJ, Mahon T, McDaid JP, Campbell J, Mano H, Brennan FM, Webster D, Foxwell BM
The Journal of experimental medicine 2003 Jun 16;197(12):1603-11

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Bruton's tyrosine kinase is a Toll/interleukin-1 receptor domain-binding protein that participates in nuclear factor kappaB activation by Toll-like receptor 4.

Jefferies CA, Doyle S, Brunner C, Dunne A, Brint E, Wietek C, Walch E, Wirth T, O'Neill LA
The Journal of biological chemistry 2003 Jul 11;278(28):26258-64

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The BTK polyclonal antibody (Cat # PAB3419) is used in Western blot to detect BTK in Ramos cell lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of BTK polyclonal antibody (Cat # PAB3419) transiently transfected HEK293 cell line lysate (1 ug/lane). BTK polyclonal antibody (arrow) was detected using the purified polyclonal antibody (1 : 1500 dilution).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with BTK polyclonal antibody (Cat # PAB3419) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)