Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 601702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#601702, RRID:AB_2715907
- Product name
- Purified anti-BTK Phospho (Tyr 223) antibody
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- A16128B
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysates (15 µg protein) from Ramos cells treated with (+) or without (-) 2 mM H2O2 for 3 minutes were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL (1:250) purified anti-BTK Phospho (Tyr 223) antibody (Clone A16128B, left) or a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (clone Poly4053) for clone A16128B, and HRP donkey anti-rabbit IgG (clone Poly4064) for the competitor’s clone, each at a 1:3000 dilution. Equal BTK loading was confirmed using a pan-BTK antibody.
- Conjugate
- Unconjugated