Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA501118 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501118, RRID:AB_11124563
- Product name
- Anti-BTK mouse monoclonal antibody, clone OTI10E10 (formerly 10E10)
- Antibody type
- Monoclonal
- Description
- Anti-BTK mouse monoclonal antibody, clone OTI10E10 (formerly 10E10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BTK
- Isotype
- IgG
- Antibody clone number
- OTI10E10
- Vial size
- 100 µl
- Concentration
- 0.87 mg/ml
Submitted references X-linked agammaglobulinemia associated with B-precursor acute lymphoblastic leukemia.
Hoshino A, Okuno Y, Migita M, Ban H, Yang X, Kiyokawa N, Adachi Y, Kojima S, Ohara O, Kanegane H
Journal of clinical immunology 2015 Feb;35(2):108-11
Journal of clinical immunology 2015 Feb;35(2):108-11
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (RC211582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-BTK monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-BTK monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BTK mouse monoclonal antibody (TA501118) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK(RC211582).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-BTK mouse monoclonal antibody (TA501118).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501118, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometry analysis of monocytes from non-XLA or XLA patients using anti-BTK antibody (TA501118). Image courtesy: Dr. H. Kanaegane, University of Toyama, Japan.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Flow Cytometry analysis of intracellular BTK expression by using anti-BTK antibody (clone OTI10E10) in CD14+ monocytes. Black histograms indicate BTK expression and Gray histograms indicate isotype control. BTK expression in the patient was reduced compared with that in a normal control.
- Validation comment
- FC
