HPA004120

antibody from Atlas Antibodies

Targeting: TPPP2 C14orf8, CT152, p18, p25beta

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA004120

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA004120, RRID:AB_2303582

Product name

Anti-TPPP2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

TFHRFAAFGESSSSGTEMNNKNFSKLCKDCGIMDG
KTVTSTDVDIVFSKVKAKNARTITFQQFKEAVKEL
GQKRFKGKSPDEVLENIYGLMEGKDPATTGATKAT
TVGAVDRLTDTSKYTGTHKERFDESGKGKGIAGRE
EMT


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and tPPP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406282).

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and prostate tissues using Anti-TPPP2 antibody. Corresponding TPPP2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows low expression as expected.

Sample type

HUMAN