MA5-24586

antibody from Invitrogen Antibodies

Targeting: ADA ADA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24586

Provider

Invitrogen Antibodies

Product name

Adenosine Deaminase Monoclonal Antibody (CL0176)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SDNQPEGMIS ESLDNLESMM PNKVRKIGEL VRYLNTNPVG GLLEYARSHG FAAEFKLVDQ SGPPHEPKFV YQAKVGGRWF PAVCAHSKKQ GKQEAADAAL RVLIGENEKA ERMGFTEVTP VTGASLRRTM LLLSRSPEA

Antibody clone number

CL0176

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Adenosine Deaminase in Lane1: Marker (kDa), Lane 2: Human cell line U-251 MG Samples were probed using a Adenosine Deaminase Monoclonal Antibody (Product # MA5-24586).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Adenosine Deaminase Monoclonal Antibody (CL0176) (Product # MA5-24586). Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ADA in A549 cells using Adenosine Deaminase Monoclonal Antibody (CL0176) (Product # MA5-24586), showing specific staining in the nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Adenosine Deaminase in human testis tissue shows nuclear positivity. Samples were probed using a Adenosine Deaminase Monoclonal Antibody (Product # MA5-24586).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Adenosine Deaminase in human kidney tissue shows nuclear positivity. Samples were probed using a Adenosine Deaminase Monoclonal Antibody (Product # MA5-24586).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Adenosine Deaminase in human cerebral cortex tissue shows nuclear positivity. Samples were probed using a Adenosine Deaminase Monoclonal Antibody (Product # MA5-24586).