Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [2]
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- Product number
- PA5-47938 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFNGR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human IFN- gamma R1 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of recombinant Human IFN-γ reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-γ (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-γ R2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47938). The ND50 is typically 0.5-2.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IFNGR2 in Blood-derived granulocytes. Samples were incubated in IFNGR2 polyclonal antibody (Product # PA5-47938) or control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of IL-IFN-gamma is shown by decrease in OD (measure of EMCV induced cytopathy) with increasing concentrations of IFN gamma R2 polyclonal antibody (PA5-47938).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IFNGR2 in HeLa human cervical epithelial carcinoma cell line. Samples were incubated in IFNGR2 polyclonal antibody (Product # PA5-47938). Recombinant Human IFN‚γ reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN‚γ (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN‚γ R2 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.5-2.5 µg/mL.