10266-1-AP

antibody from Invitrogen Antibodies

Targeting: IFNGR2 AF-1, IFNGT1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10266-1-AP

Provider

Invitrogen Antibodies

Product name

IFNGR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AWVTMPWFQ HYRNVTVGPP ENIEVTPGEG SLIIRFSSPF DIADTSTAFF CYYVHYWEKG GIQQVKGPFR SNSISLDNLK PSRVYCLQVQ AQLLWNKSNI FRVGHLSNIS CYETMADAST ELQQVILISV GTFSLLSVLA GACFFLVLKY RGLIKYWFHT PPSIPLQIEE YLKDPTQPIL EALDKDSSPK DDVWDSVSII S (125-324 aa encoded by BC003624)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.15 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10266-1-AP (IFNGR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 10266-1-AP (IFNGR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 10266-1-AP (IFNGR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug IFNGR2 antibody (10266-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-IFNGR2 (IP:10266-1-AP, 4ug; Detection:10266-1-AP 1:500) with A549 cells lysate 1800ug.