Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Other assay [1]
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- Product number
- A300-110A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BLM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse BLM by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody (Product # A300-110A; lot A300-110A-4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BLM by western blot. Samples: Whole cell lysate (15 µg) from HEK293T, K-562, Jurkat, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody (Product # A300-110A; lot A300-110A-4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BLM antibody A300-110A (lot A300-110A-3) used for IP at 6 µg/mg lysate. BLM was also immunoprecipitated by a previous lot of this antibody (lot A300-110A-2) and A300-120A. For blotting immunoprecipitated BLM, A300-110A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse BLM by western blot. Samples: Whole cell lysate (50 µg) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-BLM antibody A300-110A (lot A300-110A-2) used for WB at 2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BLM by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-BLM antibody A300-110A (lot A300-110A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody (Product # A300-110A; lot A300-110A-4) used for IP at 6 µg per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot A300-110A-3). For blotting immunoprecipitated BLM, Product # A300-110A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.